PUF的RNA结合蛋白FBF-2可以维持干细胞,而不会与RNA结合.
Brian H Carrick1,2, Sarah L Crittenden1, MaryGrace Linsley1,3
1Department of Biochemistry, University of Wisconsin-Madison, Madison, WI.
bioRxiv : the preprint server for biology
|November 1, 2024
概括
在C. elegans中的RNA结合蛋白FBF-2即使没有RNA结合,也能维持干细胞. 蛋白质相互作用可以弥补丢失的RNA结合,挑战已建立的RBP活动模型.
科学领域:
- 分子生物学分子生物学
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 众所周知,包括C. elegans FBF-2在内的PUF蛋白通过三方识别动机 (TRMs) 结合特定RNA.
- 传统的理解是,RNA结合对RNA结合蛋白 (RBPs) 的生物活性至关重要.
研究的目的:
- 研究RNA结合在FBF-2生物活性中的作用.
- 为了确定蛋白质-蛋白质相互作用是否可以影响FBF-2活动,而不依赖于RNA结合.
主要方法:
- 产生和分析一种不能结合RNA的FBF-2突变蛋白.
- 对突变FBF-2在维护C. elegans的干细胞中的生物活性进行评估.
- 评估FBF-2与伙伴蛋白的相互作用.
主要成果:
- 一个缺乏RNA结合能力的FBF-2突变体在生物学上保持活跃,并成功地维持了干细胞.
- 当它与伙伴蛋白的相互作用弥补了RNA结合的损失时,FBF-2的生物活性被保留了.
- 只有当RNA结合和伴侣蛋白相互作用都受到损害时,才会完全丧失生物活性.
结论:
- 对于所有RBP的生物活性来说,RNA结合并不是严格必要的,这挑战了现有的范式.
- 蛋白质与蛋白质的相互作用可以在功能上替代像FBF-2这样的RBP中的RNA结合.
- RBPs的活性是由RNA结合和蛋白质合作伙伴相互作用的组合调节的.
相关概念视频
Regulation of Hematopoietic Stem Cells
3.1K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.1K
Maintenance of the ES Cell State
2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K
Multipotency and Niche of Bulge Stem Cell
3.4K
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
3.4K
Stem Cell Niche
5.0K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.0K
Transcriptional Regulation: Riboswitches
3
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
3
Embryonic Stem Cells
3.4K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
3.4K


