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Updated: Jun 8, 2025

Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells
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Engineering Oncogenic Heterozygous Gain-of-Function Mutations in Human Hematopoietic Stem and Progenitor Cells

Published on: March 10, 2023

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替代性转录重新编码人类基因以表达重叠的,框架移动的微蛋白质

Haomiao Su1,2, Samuel G Katz3, Sarah A Slavoff1,2,4

  • 1Department of Chemistry, Yale University, New Haven, CT 06520, USA.

bioRxiv : the preprint server for biology
|November 1, 2024
PubMed
概括

人类基因可以通过替代转录来表达来自内部开放阅读框架 (iORF) 的隐藏微蛋白. 这一发现揭示了人类转录组和蛋白质组的新复杂性,挑战了以前关于基因表达的假设.

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The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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