通过Pyrogallol Red-Molybdate方法确定尿蛋白总量的酸盐的干扰和消除
Shijian Fan1, Jiaqing Liu1, Yu Wei1
1Department of Clinical Laboratory, The Second Affiliated Hospital of Anhui Medical University, Hefei city, 230022, China.
Practical laboratory medicine
|November 1, 2024
概括
高尿酸亚酸盐水平可以干扰使用Pyrogallol红酸盐 (PRM) 方法测量尿蛋白总量. 酸有效地扭转了这种干扰,确保了精确的蛋白质检测.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 尿液分析 尿液分析
背景情况:
- 尿蛋白水平是关键的诊断标志物.
- 皮洛加红酸盐 (PRM) 方法通常用于尿道总蛋白质的量化.
- 通过PRM方法观察到的负蛋白值与尿中高酸盐水平相关.
研究的目的:
- 用PRM方法研究酸盐对尿道总蛋白质测量的干扰.
- 评估L-亚斯科布酸在减轻亚酸干扰方面的有效性.
主要方法:
- 使用PRM方法对120个尿样进行了总蛋白质分析.
- 为了评估干扰,添加了不同度的亚酸盐 (2-200 mg/L).
- 测试了不同度的L-亚斯科布酸 (100-2000毫克/升),以逆转干扰.
主要成果:
- 亚酸盐度为10 mg/L及以上显著影响蛋白质测量.
- 在200 mg/L时,亚酸盐可降低检测到的蛋白质水平高达89.33%.
- 500 mg/L L-亚斯科布酸有效消除了化物干扰,而不会影响蛋白质检测.
结论:
- 亚酸盐干扰了PRM方法检测尿道总蛋白质的过程.
- 酸可以抵消亚酸干扰,恢复精确的蛋白质测量.
- 这一发现对于可靠的尿蛋白诊断至关重要.
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