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聚乙烯微塑料会影响Drosophila模型中的行为,氧化应激和分子反应

Himanshu Ranjan¹, Swetha Senthil Kumar¹, Sharine Priscilla¹

¹Department of Biotechnology, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology, Kattankulathur-603203, India. sahabuds@srmist.edu.in.

Environmental science. Processes & impacts

|November 1, 2024

概括

聚乙烯微塑料 (PE MPs) 伤害果,导致行为问题和氧化应激. 这项研究表明,PE MPs破坏运动功能,并激活Drosophila的细胞应激反应.

科学领域:

环境毒理学环境毒理学
生态毒理学生态毒理学
分子生物学分子生物学

背景情况:

微塑料污染是一个日益严重的全球性问题,对野生动物和人类健康的潜在影响.
聚乙烯微塑料 (PE MP) 是普遍存在的环境污染物.
调查PE MPs的毒理效应对于了解其环境风险至关重要.

研究的目的:

评估聚乙烯微塑料 (PE MPs) 对模型生物Drosophila melanogaster*的毒理影响.
评估PE MPs对果行为参数,氧化应激标志物和基因表达的影响.

主要方法:

在口服中, *Drosophila melanogaster* 暴露于PE MPs 的剂量为1 mg/mL和10 mg/mL.
评估爬和爬行行为.
测量反应性氧物种 (ROS),超氧化脱酶 (SOD) 和谷氨-S转移酶 (GST) 的活性.
使用RT-qPCR对*Hsp70Bc*,*rpr*和*p53*基因表达的分析.

主要成果:

在成年和幼虫中分别观察到爬和爬行活动的显著减少.
在幼虫和成人的阶段,ROS水平升高表明氧化应激.
减少SOD活动和GST活动的度依赖性增加,表明异生菌代谢的激活.
*Hsp70Bc*, *rpr* 和 *p53* 基因的升调,表明细胞死亡和应激反应通路的激活.

结论:

聚乙烯微塑料诱导行为障碍和氧化应激 *Drosophila melanogaster*.
PE MPs触发细胞应激反应,并激活细胞死亡途径.
这项研究突出了PE MPs对模型生物的有害毒理影响,强调了环境健康方面的担忧.

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