通过C SABRE在乙/水混合物中进行过极化[2-13C]-pyruvate
Oksana A Bondar1, Gamal A I Moustafa2, Thomas B R Robertson1
1School of Chemistry, Highfield Campus, Southampton, SO17 1BJ, UK. t.b.r.robertson@soton.ac.uk.
The Analyst
|November 1, 2024
概括
通过可逆交换信号放大 (SABRE) 在酸盐/水溶剂中超极化pyruvate,为增强的NMR和MRI应用提供了替代甲醇的可行替代品.
科学领域:
- 核磁共振 (NMR) 光谱学是指核磁共振 (NMR) 的光谱学.
- 磁共振成像 (MRI) 是一种磁共振成像技术.
背景情况:
- 通过可逆交换信号放大 (SABRE) 增强了NMR信号,使用了.
- 酸盐是超极化NMR和MRI的关键基质.
- 通常在SABRE中使用的甲醇-d4不适合体内应用.
研究的目的:
- 开发一种SABRE方法,在溶剂中超极化pyruvate,与体内研究相容.
- 探索乙/水混合物作为甲醇的替代品,用于pyruvate超极化.
- 调查影响新溶剂系统信号增强的因素.
主要方法:
- 使用SABRE进行[2-13C]pyruvate和[1-13C]pyruvate的高极化.
- 使用室温的乙/水溶剂系统.
- 检测到的NMR信号与一个1.1 T台式NMR光谱仪.
- 研究了催化剂度,DMSO和溶剂成分对信号增强的影响.
主要成果:
- 在乙/水中成功超极化[2-13C]酸盐和[1-13C]酸盐.
- 证明了室温SABRE超极化作为甲醇的替代品.
- 报告了[2-13C]-pyruvate溶液的放松时间,以指导未来的净化.
结论:
- SABRE在乙/水中的pyruvate的超极化是可行的.
- 这种方法为体内应用提供了一个有前途的替代溶剂系统.
- 进一步的研究可以建立在这些发现的基础上,以改进基于酸盐的MRI技术.
更多相关视频
11:57Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate
Published on: September 13, 2019
6.5K
14:56Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy
Published on: April 21, 2023
1.4K
相关概念视频
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
1.0K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.0K
¹³C NMR: ¹H–¹³C Decoupling
1.0K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.0K
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)
263
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
263
Double Resonance Techniques: Overview
191
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
Spin decoupling is usually achieved by...
191
