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Updated: Jun 8, 2025

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Published on: May 27, 2014

肝硬化患者的新陈代谢差异使用Q精确的混合四极轨道轨道质谱技术

Ying Xiao¹, Jie Lu², Suyan Xu³

¹Department of Infectious Diseases, Hebei Medical University Third Hospital, Shijiazhuang 050051, China.

Journal of proteome research

|November 1, 2024

概括

肝硬化 (LC) 患者的代谢物水平可以表明疾病的严重程度. 已识别的六种特定代谢物可能有助于确定LC阶段,并预测急性至慢性肝衰竭 (ACLF) 的发展.

科学领域:

代谢学代谢学代谢学
肝病学肝病学是一种肝病学.
生物标志物发现发现

背景情况:

肝硬化 (LC) 的进展与增加住院和死亡率有关.
对于及时和有针对性的干预来说,精确的LC分期至关重要.
现有的生物标志物可能无法完全捕捉LC严重性的动态变化.

研究的目的:

为了确定与肝硬化不同阶段相关的特定血清代谢物.
评估这些代谢物的潜力,作为疾病进展和急性至慢性肝衰竭 (ACLF) 的生物标志物.
利用先进的质谱仪进行全面的新陈代谢分析.

主要方法:

来自139名LC或ACLF患者的血清样本使用超高性能液态染色学-Q Exactive混合四极管-轨道管质谱法 (UPLC-Q-Exactive) 进行分析.
使用短时间序列表达式矿工 (STEM) 和丰富分析选和分析差异积累代谢物 (DAM).
进行了单变量和多变量分析,以确定显著的DAM及其与疾病严重程度 (Child-Turcotte-Pugh得分) 和并发症的相关性.

主要成果:

在患者群体中发现了9种显著的DAM.
在STEM分析中,突出了6种代谢物,包括8'-基亚布西西酸,HDCA,酸-3-酸盐,印度斯皮辛,埃普莱伦和DEHP,其水平在儿童-图尔科特-皮格B类达到峰值,然后下降.
关于LC并发症和患者子组,观察到8'-氧亚布西西酸,eplerenone和DEHP水平的显著变化.

结论:

六种已识别的DAM的血清水平显示出在肝硬化中表征代谢变化的潜力.
这些代谢物可以作为确定LC严重性的有价值指标.
鉴定到的代谢物可以帮助预测ACLF的发展,从而能够更早地进行临床干预.

关键词:

孩子-图尔科特-普格在 UPLC-Q-Exactive 中使用.肝硬化是肝硬化的一种疾病.代谢生物组的代谢生物组

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