"在埃及的儿科 Shigella 隔离物中扩展光谱β-乳糖酶耐药性的分子表征"
Dina F Badr1, Maysaa El Sayed Zaki2, Ahmed Gomaa Elsayed1
1Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Biochemical genetics
|November 1, 2024
概括
在患有腹的儿童的Shigella分离物中发现了抗生素耐药性和扩展谱β-乳糖酶 (ESBL) 耐药性的高率. 这凸显了控制滥用抗生素和监测耐药性模式的迫切需要.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 公共卫生 公共卫生
背景情况:
- 石病是导致儿童发病和死亡的重要原因,特别是在发展中国家.
- 滋格拉菌中扩展谱β-乳糖酶 (ESBL) 耐药性的上升使有效的治疗策略复杂化.
研究的目的:
- 调查来自患有急性腹的儿科患者的Shigella分离物的抗生素耐药性模式和ESBL概况.
主要方法:
- 细菌学测试,血清型定型和多重PCR被用于Shigella的识别.
- 通过磁盘扩散试验评估了抗微生物耐药性.
- 现型检测和PCR检测确定了ESBL和特定基因 (CTX-M,SHV,TEM).
主要成果:
- 确定了100种Shigella分离物 (10%的患病率),其中S. sonnei和S. dysenteries是最常见的.
- 观察到对安皮西林 (100%) 的完全耐药性和对西普罗夫洛克萨 (24%) 的显著耐药性;没有对伊米佩内姆的耐药性.
- 54%的分离物体表现出ESBL的产生,其中 bla-CTX-M (50%) 和 bla-TEM (48.1%) 是主要检测到的基因.
结论:
- 该研究揭示了西格拉菌中抗生素和ESBL耐药性的惊人率,强调了限制未经指导的抗生素使用的关键需要.
- 持续监测局部和全球抗生素耐药性趋势对于制耐药性传播至关重要.
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