在模拟组织模型中对药物的定量分析,使用三极四极质谱仪上的纳米-DESI
Alyssa M Moore1, Andrew Bowman2, Syeda Nazifa Wali1
1Department of Chemistry, College of Science, Purdue University, West Lafayette, Indiana 47907, United States.
Journal of the American Society for Mass Spectrometry
|November 1, 2024
概括
定量质谱成像 (MSI) 对于药物分布研究至关重要. 这项研究表明,使用模拟组织模型的气压矩阵辅助激光脱/电离 (AP-MALDI) 与大气压矩阵辅助激光脱/电离 (AP-MALDI) 相比,纳米喷雾脱电喷雾电离 (纳米-DESI) 提供了更高的量化和检测极限.
科学领域:
- 分析化学 分析化学
- 药品分析 药品分析
背景情况:
- 质谱成像 (MSI) 对于可视化生物样本中药物分布至关重要.
- 量化MSI受到复杂矩阵和缺乏先前分离的挑战.
- 模拟组织模型通过创建矩阵匹配的校准曲线来帮助定量MSI.
研究的目的:
- 评估和比较纳米喷涂脱电喷电离 (纳米-DESI) 和大气压矩阵辅助激光脱/电离 (AP-MALDI) 的定量性能.
- 评估模拟组织模型对定量MSI的实用性,使用多重反应监测 (MRM) 模式中的三重四极质谱仪 (QqQ).
主要方法:
- 利用模仿组织模型,在组织同质物中使用不同度的分析物.
- 采用纳米-DESI和AP-MALDI技术进行质谱成像.
- 在多重反应监测 (MRM) 模式下使用三重四极质谱仪 (QqQ) 进行量化.
主要成果:
- 与AP-MALDI相比,纳米-DESI对测试化合物的标准偏差较低.
- 纳米-DESI实现了研究分析物的超级检测极限.
- 该研究确定了与模拟组织模型实施相关的局限性和挑战.
结论:
- 由于其精度和灵敏度的提高,纳米-DESI对定量质谱成像具有显著的前景.
- 模拟组织模型是一种可行的,虽然不适用于所有人的方法,用于增强MSI量化.
- 需要进一步的研究来克服模仿组织模型对各种分析物的局限性.
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