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通过单个菌株元编码分析,对有害藻类繁殖物种Phaeocystis globosa的基因内变异

Yifei Wang¹, Nansheng Chen²

¹CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao 266200, China; College of Marine Science, University of Chinese Academy of Sciences, Beijing 100039, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China.

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概括

在Phaeocystis globosa 18S rDNA V4中高的基因内变异 (IGV) 可以扭曲元编码结果. 了解这些变异对于准确的物种和遗传多样性评估至关重要.

科学领域:

微生物生态学微生物生态学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

基因内变异 (IGV) 可以使生物多样性评估使用metabarcoding复杂化.
18S rRNA基因的V4区域通常用于元编码,但其内部的IGV尚未完全理解.

研究的目的:

调查IGVs在Phaeocystis globosa的18SrDNAV4区域中的存在和影响.
确定IGV是否会影响P. globosa种群中遗传多样性的解释.

主要方法:

对54个Faeocystis globosa菌株进行了元编码分析.
在18SrDNAV4区域内对AMplicon序列变异 (ASV) 的分析.
对不同菌株和现场样本的ASV概况进行比较.

主要成果:

每个P. globosa菌株都包含在18SrDNA V4区域的200多个不同的ASV.
主导的ASV在菌株之间有所不同,这表明了高遗传多样性和IGV的存在.
跨菌株共享的ASV证实了IGV是真正的生物变异,而不是测序文物.
现场样本的元编码还揭示了许多IGV.

结论:

全球菌在它的18S rDNA V4区域表现出显著的基因内变异.
这些IGV可以导致在metabarcoding研究中高估物种和遗传多样性.
准确解释元编码数据需要考虑IGVs,可能是由于不完整的同质化或伪基因.

关键词:

18S rDNA V4 的时间在基因内变化的变化.分析元编码的分析.囊 (Phaeocystis globosa) 是一个全球性植物.

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