低温下管的基底真空化:基于79例法医解剖病例的综合病理研究
Shojiro Ichimata1, Yukiko Hata1, Naoki Nishida1
1Department of Legal Medicine, Faculty of Medicine, University of Toyama, Toyama, Japan.
Forensic science international
|November 1, 2024
概括
组织病理学检查,特别是H&E染料中的基底真空化 (BV),是诊断低温病例中酸性脂肪酸症的关键. 先进的染色方法提高了检测,但需要对法医病理学的仔细解释.
科学领域:
- 法医病理学 法医病理学
- 组织病理学 组织病理学
- 毒理学 毒理学 毒理学
背景情况:
- 酸性脂肪酸症是低温症的一个严重并发症.
- 组织病理学发现,如基底真空化 (BV),可以表明酸性脂肪酸症.
- 在低温病例中区分酸性症存在诊断挑战.
研究的目的:
- 评估在低温症中检测酸性脂肪酸的组织病理学检查的诊断效用.
- 为了比较标准的H&E染色与先进的脂质染色和BV检测的免疫组织化学.
- 为了将组织病理学发现与血清β-基酸盐水平相关联.
主要方法:
- 对79例低温病例的分析,包括血素和素 (H&E) 染色.
- 氧化后固定和阿迪波菲林免疫组织化学 (ADFP-IHC) 的应用用于脂质染色.
- 在75个案例中测量血清β-基酸盐水平.
主要成果:
- 基底真空化 (BV) 在H&E的30/79例和先进染色的43/79例中被确定.
- 37/75例患者血清β-基酸盐 (>1000μmol/L) 的升高.
- H&E BV阳性病例在很大程度上与血清性酸相关,但多次染色显示了差异.
结论:
- 在法医病理学中,在怀疑酸性脂肪酸症时,H&E中明显的BV模式至关重要.
- ADFP-IHC有助于确认脂质滴滴的存在.
- BV阳性受到队列人口统计的影响,需要进行区域评估.
相关概念视频
Decreased Body Temperature
602
A decreased body temperature can occur in patients with hypothermia and frostbite. Heat loss with extended cold exposure overpowers the body's ability to create heat, resulting in hypothermia. Core temperature readings help classify hypothermia. Mild hypothermia is temperatures between 32 °C (89.6 °F) and 35°C (95 °F) and is caused by impaired thermoregulation. Moderate hypothermia is temperatures between 28 C (82.4 °F) and 32 °C (89.6 °F) caused by...
602
Renal Tubule and Collecting Duct
818
The renal tubule is divided into three parts: the proximal convoluted tubule (PCT), the Loop of Henle (LOH), and the distal convoluted tubule (DCT).
Proximal Convoluted Tubule (PCT):
The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
Proximal Convoluted Tubule (PCT):
The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
818


