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优化,结构特征和生物应用由菌菌产生的外聚糖体 KT99002828 KT990028产生的外聚糖体

Leandro Paes de Brito¹, Elaine Cristina da Silva², Leandro Fragoso Lins²

¹Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235 - Cidade Universitária, 50670-901, Recife, Pernambuco, Brazil.

International journal of biological macromolecules

|November 1, 2024

概括

菌 (Enterococcus faecium KT990028) 产生了一种具有显著抗氧化和抗菌性能的新型外聚糖 (EPS). 这种功能性生物聚合物证明了食品和制药应用的安全性和潜力.

科学领域:

微生物学微生物学
生物化学生物化学
材料科学材料科学材料科学

背景情况:

外聚糖 (EPS) 是微生物聚合物,具有多种应用.
菌株是已知的生物活性化合物的生产者.
优化EPS生产并描述其特性对于潜在的应用至关重要.

研究的目的:

为了识别和优化最好的外聚糖 (EPS) 生产Enterococcus菌株.
描述所选EPS的物理化学和生物特性.
评估EPS在食品和制药行业的潜在应用.

主要方法:

对11种Enterococcus菌株进行了针对EPS生产的选.
优化发酵条件 (糖,酵母提取物,脱脂牛奶,温度,时间).
使用HPAEC,FTIR,1H NMR,SEM,DLS,X射线衍射和TGA进行表征.
对抗氧化剂,抗微生物,抗菌膜和细胞毒性活动的评估.

主要成果:

在优化条件下选择了Enterococcus faecium KT990028,在优化条件下产生2.880g/L EPS.
EPS是一种异多聚糖 (MW 166.98 kDa),具有独特的微观结构和无形/晶体性质.
显示出显著的抗氧化,抗微生物和抗菌膜活性,对Vero细胞没有细胞毒性.

结论:

来自E. faecium KT990028的EPS是一种安全且功能性的生物聚合物.
它的特征性质表明,它有可能成为食品和制药产品的有价值成分.
对其特定应用的进一步研究是有必要的.

关键词:

生物活动是生物活动.细胞毒性细胞毒性肠球菌 (Enterococcus faecium) 是一个有毒的菌株.在外聚糖类中含有外聚糖类.优化优化优化优化结构性表征结构性表征

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