用Mucor circinelloides的代谢工程来改善阿斯塔克桑的生产
Tahira Naz1, Tariq Saeed1,2, Samee Ullah1,3
1Colin Ratledge Center for Microbial Lipids, College of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China.
World journal of microbiology & biotechnology
|November 2, 2024
概括
这项研究设计了Mucor circinelloides,以产生高水平的抗氧化素颜料阿斯塔山丁. 用代谢前体补充剂显著提高了阿斯塔丁产量和生物质增长,克服了关键的生产瓶.
科学领域:
- 生物技术是生物技术.
- 微生物工程 微生物工程
- 自然产品的合成自然产品的合成
背景情况:
- 阿斯塔丁是一种有价值的抗氧化剂颜料,具有广泛的工业和健康应用.
- 粘菌 (Mucor circinelloides) 是一种用于胡卜素生成的真菌模型生物,也是一种潜在的胡卜素生产平台.
研究的目的:
- 为了设计一种复合的Mucor circinelloides菌株,以提高阿斯塔山丁的产量.
- 通过共同表达关键基因和补充代谢前体来优化阿斯塔桑产量.
主要方法:
- 在M. circinelloides中,来自Haematococcus pluvialis的编码子优化的β-胡卜素氧酶 (crtR-B) 和酶 (bkt) 基因的同时表达.
- 使用gpd1和zrt1促进器进行基因表达.
- 通过RT-qPCR评估了阿斯塔山丁的产生和基因表达;评估了谷氨酸和氨酸补充剂的影响.
主要成果:
- 再组合的M. circinelloides Mc-57菌株产生了创纪录的135μg/g的阿斯塔山丁.
- 补充谷氨酸和氨酸增加了阿斯塔克桑产量至281μg/g,并改善了生物质.
- 成功过度表达crtR-B和bkt基因与增加的阿斯塔克桑积累相关.
结论:
- 工程M. circinelloides是一种可行的生物工厂,用于高产量的阿斯塔山丁生产.
- 代谢前体补充有效地平衡了阿斯塔克桑的积累与生物质的增长.
- 这项工作为在真菌阿斯塔克桑合成方面进一步的生物技术进步提供了基础.
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