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Updated: Jun 12, 2025

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为扫描电子显微镜准备藻细胞样本和相关细菌联盟的综合性协议

Margaux Maillard1, Nicolas Stephant2, Atsuko Tanaka3

  • 1Nantes Université, CNRS, US2B, UMR 6286, 44000 Nantes, France.

STAR protocols
|November 2, 2024
PubMed
概括

这项研究详细介绍了一种方法,用于为扫描电子显微镜准备模型藻 (Phaeodactylum tricornutum) 及其相关细菌. 适当的样本保存是观察细胞结构和相互作用的关键.

关键词:
微生物学 微生物学显微镜的使用方法模型生物模型生物

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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