协议研究人类肠道细菌群落和节律性ex vivo使用化学定位系统
Helena Heimes1, Deborah Häcker2, Hélène Omer1
1Chair of Nutrition and Immunology, TUM School of Life Sciences, Technical University of Munich, 85354 Freising, Germany.
STAR protocols
|November 2, 2024
概括
这项研究详细介绍了使用化学定位系统培养人类肠道微生物的协议. 该方法可以模拟婴儿或成人结肠状况,以研究微生物社区的变化.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 系统生物学 系统生物学
背景情况:
- 肠道微生物群落在人类健康中起着至关重要的作用.
- 培养复杂的肠道微生物群ex vivo对于机理学研究至关重要.
- 现有的方法可能无法完全捕捉体内条件的动态.
研究的目的:
- 提出一个详细的协议,以建立和维护人类肠道微生物群落在化学定位系统.
- 为了实现婴儿和成人结肠环境的模拟.
- 为了促进对微生物社区对饮食干预措施的反应的调查.
主要方法:
- 从人类便样本中的细菌转移到化学定位生物反应器中.
- 优化介质组成,以支持复杂微生物生长.
- 实施16S rRNA amplicon测序用于社区分析.
- 使用昼夜节律建模分析细菌的丰富性.
主要成果:
- 成功培育出复杂的肠道微生物群落.
- 建立不同的微生物概况,模拟婴儿和成人结肠状况.
- 证明该协议在研究微生物对饮食成分反应的转变中的实用性.
结论:
- 本次提出的化学定位方案为研究人类肠道微生物群的实体研究提供了一个强大的平台.
- 该方法允许对微生物群落动态和代谢物产生进行受控的调查.
- 该协议可适应模拟不同的生理条件和饮食暴露.
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