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在雌性小鼠中,NAGK调节了青春期的开始

Wei Zhang¹, Ping Qin¹, Mengxian Li¹

¹Anhui Provincial Laboratory of Animal Genetic Resources Protection and Breeding, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui, 230036, China; Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui, 230036, China.

|November 2, 2024

概括

通过调节关键基因和激素,N-乙糖氨酸激酶 (NAGK) 在雌性小鼠中开始青春期发挥着至关重要的作用. NAGK的镇压延迟了青春期的开始,影响了生殖发育.

科学领域:

生殖生物学生殖生物学
内分泌学在内分泌学.
分子生物学分子生物学

背景情况:

青春期的开始涉及下丘脑-垂体-卵巢 (HPO) 轴内的复杂相互作用.
调节青春期开始的精确分子机制尚未完全理解.

研究的目的:

调查N-乙糖氨酸激酶 (NAGK) 在雌性小鼠中开始青春期的作用.
在开发过程中确定HPO轴中NAGK的表达模式.
评估Nagk基因淘汰对青春期相关标志物和发病的影响.

主要方法:

实时定量逆转录聚合酶连锁反应 (RT-qPCR) 和免疫光用于NAGK表达分析.
在体内和体外的研究评估基因表达,细胞增殖/细胞亡,激素水平和青春期的开始.
在小鼠中用于nagk基因淘汰的脑内 (ICV) 注射.

主要成果:

在雌性小鼠发育过程中,Nagk mRNA在下丘脑,垂体和卵巢中表达,在不同阶段的不同水平.
纳克在GT1-7细胞中的敲击降低了Gnrh和Kiss1的表达和GnrH的分泌,同时增加了亡.
在小鼠中,nagk基因被淘汰延迟了阴道开口,降低了下丘脑Gnrh和Kiss1mRNA,降低了血清雌激素 (E2) 水平.

结论:

NAGK对于调节Gnrh和Kiss1mRNA表达和GnrH分泌至关重要.
NAGK会影响下丘脑基因表达和血清E2度.
淘汰Nagk延迟了雌性小鼠的青春期开始,突出了它在生殖成熟中的关键作用.

关键词:

女性老鼠的雌性小鼠.在GT1-7单元格中,选择GT1-7单元格.敲击击退役的情况是这样的.这就是NAGKKK的意思.青春期青春期青春期

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