相关实验视频

Updated: Jun 30, 2026

Purification of Mitochondria from Yeast Cells
10:39

Purification of Mitochondria from Yeast Cells

Published on: August 24, 2009

25.1K

在酵母表达系统中大规模净化线粒体蛋白质复合体,用于结构分析

Nanako Kobayashi1, Yuta Imai1, Shintaro Kita1

  • 1Department of Clinical Laboratory Science, Division of Health Sciences, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa, Japan.

Methods in enzymology
|November 2, 2024
PubMed
概括

研究人员介绍了净化线粒体蛋白质复合体的新方法,这对于使用冷电子显微镜确定它们的结构和回答生物学问题至关重要.

关键词:
线粒体制备的线粒体制备线粒体蛋白质是线粒体蛋白质.多基因表达的多基因表达.蛋白质复合物的净化净化.结构分析 结构分析酵母表达系统是酵母表达系统.

更多相关视频

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method10:36

Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method

Published on: July 27, 2016

9.5K
Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe08:08

Analysis of the Expression and Complexes Assembly of the Mitochondrial Respiratory Chain Proteins in the Fission Yeast Schizosaccharomyces pombe

Published on: May 2, 2025

187

相关概念视频

Bacterial Protein Maturation01:26

Bacterial Protein Maturation

Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...