相关实验视频
Updated: Jun 8, 2025

10:25
Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
4.8K
VRF1的替代拼接作为一个分子开关来调节压力诱导的早期开花
Mo-Xian Chen1, Yuan Tian1, Fu-Yuan Zhu1
1State Key Laboratory of Tree Genetics and Breeding, the Southern Modern Forestry Collaborative Innovation Center, Key Laboratory of State Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, College of Life Sciences, Nanjing Forestry University, Nanjing, China.
Cell reports
|November 3, 2024
概括
植物使用一种叫做VRF1的因子来早期开花,避免干旱压力. 它的不同形式作为一个分子开关,由环境条件控制,以管理这种过渡.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 植物通过早期开花来逃避极端的环境压力.
- 控制这种转变的分子机制尚未完全理解.
研究的目的:
- 确定调节从植物生长到生殖生长的转变的基因.
- 研究VRF1在脱离干旱和避免压力的作用.
主要方法:
- 突变查以确定VRF1.1. 的突变.
- 对VRF1替代拼接和蛋白质异型 (VRF1-AS1,VRF1-AS3) 的分析.
- 研究VRF1异型与MKK1和下游信号通路的相互作用.
主要成果:
- 一种新型突变物,VRF1,显示干旱逃生功能受损.
- 通过替代拼接生成两个功能VRF1异型,VRF1-AS1和VRF1-AS3.
- 异形比,而不是绝对丰度,作为一个分子开关的开花过渡.
- VRF1异型具有竞争性地结合MKK1,激活不同的调节通路.
- 通过过早开花来避免压力,VRF1的作用在66种阿拉比多普西斯生态型中得到保护.
结论:
- VRF1是植物对环境压力反应的关键调节者.
- 调节VRF1异形比率允许精确适应动态环境变化.
- 这种机制使植物能够通过及时开花来避免压力.
相关概念视频
Alternative RNA Splicing
21.0K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.0K
RNA Splicing
56.1K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.1K
Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
Pre-mRNA Processing: RNA Splicing
5.2K
5.2K
Transcriptional Regulation: Riboswitches
3
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
3
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K

