AP3M2:神经系统的一个关键调节器调节了结直肠癌中自的过程
Maguie El Boustani1, Nayla Mouawad2, Monah Abou Alezz3
1Nephrology and Dialysis Unit, Genomics of Renal Diseases and Hypertension Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
Tissue & cell
|November 3, 2024
概括
针对AP3M2基因,在蛋白质贩运中至关重要,对结直肠癌 (CRC) 治疗有希望. 抑制AP3M2通过影响自和活性氧物种 (ROS) 来降低CRC细胞活力.
科学领域:
- 分子瘤学分子瘤学
- 癌症基因组学 癌症基因组学
- 细胞生物学 细胞生物学
背景情况:
- 结肠直肠癌 (CRC) 是全球癌症相关死亡的主要原因.
- 像"癌症基因组图谱"中的分子分析,对于识别CRC亚型中的治疗点至关重要.
- 在CRC中AP3M2基因的作用在很大程度上未被探索,尽管它已知在蛋白质贩运中的功能.
研究的目的:
- 研究AP3M2基因作为结直肠癌治疗点的潜力.
- 为了阐明AP3M2在CRC细胞系中的功能意义.
- 探索CRC中AP3M2表达,自和活性氧物种 (ROS) 之间的关系.
主要方法:
- 利用RNA干扰 (RNAi) 来使CRC细胞系中的AP3M2基因失活.
- 评估了AP3M2淘汰对HCT-116,CACO2和HT29细胞系细胞活性的影响.
- 分析了AP3M2表达,自相关基因和ROS水平之间的相关性.
主要成果:
- 发现AP3M2在结直肠癌细胞系中被放大.
- 抑制AP3M2显著降低了多个CRC细胞系的活力.
- AP3M2表达与与自相关的基因表达和ROS水平的变化相关.
结论:
- 在结直肠癌中,AP3M2充当潜在的瘤基因.
- 准AP3M2可能为CRC提供一种新的治疗策略.
- 自-ROS途径与AP3M2影响CRC进展的机制有关.
相关概念视频
mTOR Signaling and Cancer Progression
3.7K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.7K
Autophagy
4.2K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.2K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
PI3K/mTOR/AKT Signaling Pathway
3.4K
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...
3.4K
Autophagic Cell Death
3.4K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.4K
Delivery Pathways to the Lysosome
6.2K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.2K


