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由ISO上调的BECN1特别促进LC3B依赖的自和在侵袭性膀癌中抗癌活性

Xiaohui Hua¹, Daimin Xiang², Jiheng Xu³

¹Department of Occupational Health and Environmental Health, School of Public Health, Anhui Medical University, Hefei, Anhui, China; School of Laboratory Medicine and Life Sciences, Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.

Translational oncology

|November 3, 2024

概括

异索类原蛋白 (ISO) 通过调高BECN1蛋白来激活自,这对其抗膀癌 (BC) 作用至关重要. 这种机制涉及c-Myc/miR-613/NCL通路,为高度侵入性BC提供治疗潜力.

科学领域:

分子生物学分子生物学
癌症研究癌症研究
自自是一种自的过程.

背景情况:

伊索拉辛丁原蛋白 (ISO) 在预防和治疗膀癌 (BC) 中表现有前途.
ISO诱导的自对于其抗癌活性至关重要,但其机制尚未完全理解.
一种自蛋白BECN1在人体BC组织中降低调节.

研究的目的:

阐明ISO诱导自并产生抗癌作用的机制.
研究BECN1在ISO介导的自和BC抑制中的作用.
通过ISO识别负责BECN1上调的分子通路.

主要方法:

研究了ISO对BC细胞和小鼠模型中的BECN1表达的影响.
利用BECN1的枯竭来评估其在ISO诱导的自和抗癌活性中的作用.
进行了涉及c-Myc,miR-613和NCL的机制研究,以了解BECN1调节.

主要成果:

通过ISO处理,以剂量和时间依赖的方式对BECN1的表达进行了上调.
BECN1的枯竭取消了ISO诱导的LC3B依赖的自和抗癌效应.
通过c-Myc/miR-613/NCL轴,ISO诱导了BECN1上调,稳定了BECN1mRNA.

结论:

通过c-Myc/miR-613/NCL通路,ISO处理可以通过后转录上调BECN1.
这种BECN1上调启动LC3B依赖的自,并调解ISO的抗癌活性.
国际标准组织证明了高度侵入性膀癌 (HGIBC) 患者的治疗潜力.

关键词:

在BECN1中,BECN1是指BECN1.异索胺原原蛋白 (ISO) 是一种在 LC3B 里面,你会看到 LC3B.在NCL中,NCL是指NCL.自自是自的过程.

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