来自Armillariella tabescens mycelium的曼诺格拉克坦的结构特征和抗高尿素作用
Wenhua Guo1, Junqiang Zhang2, Hailan Deng1
1Anhui Key Laboratory for Ecological Engineering and Biotechnology, School of Life Sciences, Anhui University, Hefei 230601, Anhui, China.
International journal of biological macromolecules
|November 3, 2024
概括
来自Armillariella tabescens的天然多糖化物 (AT-W) 通过抑制山丁氧化酶和调节载体表达,有效降低小鼠的血清尿酸. 此外,AT-W还可以预防高尿血引起的损伤,显示出治疗潜力.
科学领域:
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
- 自然产品 化学 化学
背景情况:
- 超尿血症是一种 purin代谢障碍,导致并发症,需要有效的治疗,副作用最小.
- 目前治疗高尿血症的治疗选择往往具有局限性或不良影响.
研究的目的:
- 在超尿血症的小鼠模型中研究来自Armillariella tabescens的天然同质多糖体 (AT-W) 的治疗潜力.
- 阐明AT-W对尿酸代谢和脏保护的影响背后的机制.
主要方法:
- 结构特征AT-W,包括分子量测定和单糖组成分析.
- 在小鼠高尿血病模型中对AT-W的体内评估,评估血清尿酸水平,酶活性,基因表达和损伤.
- 分析AT-W对丁氧化酶活性和关键载体ABCG2,OAT1和URAT1.1的表达的影响.
主要成果:
- 平均分子量为25.6kDa的AT-W主要由银河糖,曼诺糖,糖和阿拉比诺糖组成.
- 在超尿血症小鼠中,AT-W显著降低了血清尿酸水平.
- 该机制涉及抑制桑丁氧化酶,ABCG2和OAT1的上调和URAT1的下调,以及对损伤的保护.
结论:
- 在小鼠模型中,AT-W显示出显著的高尿路血效应和对高尿路血引起的脏病的保护作用.
- 这些发现表明,AT-W具有相当大的治疗潜力,用于管理高尿血和相关并发症.
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