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基于高分子聚合物的HPLC静态阶段:绿色合成策略和有前途的应用前景

Qiaoling Liu¹, Wanjiao Wei¹, Yanjuan Liu²

¹School of Chemistry and Environmental Engineering, Hubei Key Laboratory of Novel Reactor and Green Chemical Technology, Wuhan Institute of Technology, Wuhan, 430205, China.

Analytica chimica acta

|November 3, 2024

概括

研究人员开发了一种新型的深度欧特克超分子聚合物 (DESP) HPLC静止阶段. 这种绿色合成方法可以有效地分离各种分析物,包括实物样本中的类固醇激素.

科学领域:

分析化学分析化学
材料科学材料科学材料科学
聚合物化学聚合物化学

背景情况:

深度浸泡溶剂 (DES) 是具有成本效益的,具有多种应用的可生物降解材料.
深度欧特克超分子聚合物 (DESP) 将DES特性与超分子聚合物特性结合起来,在分离科学中显示出潜力.
作为高性能液态染色学 (HPLC) 的静止相,DESP以前没有被探索过.

研究的目的:

为HPLC开发和描述一种基于DESP的新型静止相.
评估新静止相对各种分析物的分离能力.
证明DESP静止阶段在真实样本中的类固醇激素分析中的应用.

主要方法:

使用DES作为单体和反应介质的DESP静止相 (Poly(DES) @SiO2) 的绿色合成.
对Poly(DES) @SiO2柱的相互作用机制和对水友性和疏水性物质的分离选择性的评估.
与商业C18柱相比,对柱的效率,机械稳定性和分离可重复性的评估.

主要成果:

通过多种相互作用机制,Poly(DES)@SiO2列证明了结构相似的水友性/疏水性物质通过多种相互作用机制进行选择性分离.
与C18柱相比,疏水分析物的分离速度更快,并且需要较少的有机化剂,尽管效率略低.
该柱体表现出优异的机械稳定性和良好的可重复性,用于分离类固醇激素,实际样本的恢复率在94.56%至103.84%之间.

结论:

本研究提出了一种绿色和简单的策略,用于合成基于DESP的新型静止相.
已经验证了使用DESP进行有效的HPLC分离的可行性.
基于DESP的静止相显示了分析复杂样品的有希望的潜力,扩大了DES在分离科学中的应用.

关键词:

深层欧性溶剂深层欧性溶剂深度欧特克的高分子聚合物.液体色谱学液体色谱学液体色谱学静止阶段是静止的阶段.

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