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实验室在芯片上的基于纸张的电化学辅助固相微提取和离子选择性传感器用于在生物流体样本中测定纳普

Hanieh Haghgouei¹, Naader Alizadeh¹

¹Department of Chemistry, Faculty of Basic Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.

Analytica chimica acta

|November 3, 2024

概括

这项研究提出了一个新的实验室芯片平台,用于快速和便携式检测纳素 (NAP). 集成设备结合了电化学控制的固体相微提取 (EC-SPME) 和离子选择性电极 (ISE) 进行生物样本的有效分析.

科学领域:

分析化学分析化学
材料科学材料科学材料科学
生物医学工程生物医学工程

背景情况:

基于纸质的分析设备 (PAD) 和芯片上的实验室 (LOC) 技术使小型化,快速和低成本的诊断成为可能.
资源有限的环境显著受益于便携式和可访问的测试解决方案.

研究的目的:

开发一个集成的LOC平台,用于双重功能:电化学控制的固相微提取 (EC-SPME) 和的电位测量测定 (NAP).
使用纸质分析装置 (PAD) 进行选择性提取和检测生物样本中的NAP.

主要方法:

使用化学方法制造导电纸.
导电分子印记聚合物 (CMIP) 薄膜的电化学沉积在导电纸上,使用NAP作为模板.
在微流体芯片设备中集成EC-SPME和电位计离子选择电极 (ISE).
优化抽取和检测室的分析过程.

主要成果:

一个基于纸质的CMIP成功地制造并用于NAP的选择性EC-SPME.
集成的LOC-PAD在4.0×10−7至1.0×10−2mol L−1的度范围内对NAP表现出高选择性.
确定NAP的检测极限 (LOD) 为2.0 × 10−7 mol L−1,ISE快速响应时间为7秒.
在使用便携式设备的真实样本中,获得了令人满意的尖端恢复 (90-110%) 和较低的相对标准偏差 (RSDs ≤7%).

结论:

开发的LOC-PAD有效地实现了选择性提取和在血清和唾液中确定NAP.
EC-SPME促进了样本清理,减少了干扰,并增强了ISE的选择性反应.
在单一芯片中集成EC-SPME和ISE提供了一种便携且高效的方法,用于在有限的生物样本体积中确定NAP的微量.

关键词:

在CMIP中,CMIP是最重要的.在EC-SPME中使用.完全可以携带的便携式.在LOC-PAD系统中,使用LOC-PAD.纳普洛克森传感器的传感器是这是唾液的唾液.

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