内源性部分脱过程对乙酸和酸作为碳来源的反应:酸积累性能,微生物群体的动态变化和元基因组洞察力
Jiantao Ji1, Ying Zhao1, Guanqi Wu1
1College of Ecology and Environment, Zhengzhou University, Zhengzhou, 450001, China.
Water research
|November 3, 2024
概括
碳源影响内源部分脱 (EPD) 以有效去除. 乙酸盐通过影响微生物新陈代谢和基因表达来促进比酸盐更高的酸盐积累.
科学领域:
- 环境微生物学 环境微生物学
- 废水处理技术 废水处理技术
- 生物地质化学循环的过程
背景情况:
- 内源部分脱 (EPD) 是一种有前途的节能去除的方法,为anammox工艺提供.
- 关于不同碳来源对EPD性能和潜在代谢途径的影响的了解有限.
- 调查碳源效应对于优化EPD系统至关重要,尤其是在不同的基板条件下.
研究的目的:
- 阐明不同酸盐和酸盐比对EPD系统中酸盐到酸盐转化率 (NTR) 的影响.
- 探索受不同碳源影响的代谢机制,包括多基酸盐 (PHAs) 的储存和基因表达.
- 为管理具有复杂或缺陷碳来源的EPD系统提供见解.
主要方法:
- 在180天内运行EPD系统,在8个不同的阶段控制酸盐和酸盐的比率.
- 分析酸盐转化为酸盐的转化率 (NTR) 和量化储存的多基酸盐 (PHB/PHV).
- 进行元基因组分析,以评估微生物社区结构和功能基因丰富度,重点关注酸盐还原酶基因.
主要成果:
- 随着乙酸/酸比率转向酸,NTR显著下降,从81.7%降至0.4%,但在恢复到乙酸后恢复到86.1%.
- 乙有利于聚3-基酸盐 (PHB) 的储存,与较高的NTR相关,而酸盐导致聚3-基酸盐 (PHV) 的储存和微不足道的酸盐积累.
- 甲基因组分析显示,微生物群落稳定,但酸减少了NADH生产基因的丰富性,阻碍了酸盐的减少和酸盐的积累.
结论:
- 乙酸与酸的比率通过改变PHA储存和微生物代谢途径,极大地影响EPD性能.
- 与酸盐相比,酸盐限制了NADH的可用性,从而降低了酸盐减少和随后酸盐积累的效率.
- 结果为优化EPD系统中的碳源策略提供了指导,以提高去除效率.
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