使用高分辨率质谱仪在母体血清中溶解内源和外源化学物质
Akifumi Eguchi1, Kenichi Sakurai1, Midori Yamamoto1
1Chiba University, Center for Preventive Medical Sciences, Inage-ku Yayoi-cho 1-33, Chiba, Japan.
Ecotoxicology and environmental safety
|November 3, 2024
概括
这项研究使用人类血清的非向分析来确定环境化学物质暴露及其代谢影响. 这些发现将化学物质暴露与新陈代谢的改变联系起来,突出了暴露组研究的新方法.
科学领域:
- 环境化学环境化学
- 代谢学 代谢学 代谢学
- 人类生物监测 人类生物监测
背景情况:
- 越来越多的环境化学物质暴露需要先进的分析方法来检测人类样本中的未知物质.
- 了解外源化学物质暴露和内源代谢特征之间的相互作用对于人类健康评估至关重要.
研究的目的:
- 开发和应用一种新的分析方法来将化合物分类为人体血清中的内源性或外源性.
- 调查已识别的化学暴露标志物与孕妇代谢模式之间的关系.
- 探索PubChemLite for Exposomics数据库在全面的暴露组研究中的实用性.
主要方法:
- 来自84名孕妇的血清样本使用液体染色学-四极点飞行时间质谱法 (LC-QToFMS) 进行分析.
- 化合物使用PubChemLite for Exposomics数据库进行注释和分类.
- 多变量统计方法包括主要组件分析 (PCA),规范化通用法定相关性分析 (rGCCA) 和统一多重近似和投影 (UMAP) 用于模式分析.
- 使用OPTICS集群来识别不同的复合模式组.
主要成果:
- 确定并分类了106种化合物 (51种内源,55种外源),包括食物质,酸盐和类.
- 通过缩小维度和集群,通过显著的复合重叠,揭示了两个不同的集群.
- 路径丰富分析表明,化学物质暴露与氨基酸代谢,蛋白质/矿物质运输和能量代谢的改变之间存在关联.
结论:
- 该研究提出了一种新的综合方法,通过从单一数据集中分析外源和内源化学物质来进行暴露组研究.
- 虽然观察到化学物质暴露和代谢变化之间的关联,但无法确定因果关系.
- 这些发现有助于了解在人类生物监测中多重化学物质暴露的代谢影响,尽管样本大小和注释准确性的限制.
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