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Updated: Jun 8, 2025

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长非编码RNAVENTHEART对于心室心肌细胞的特异性和功能是必需的

Yiqing Yang¹, Albert Dashi², Poh Loong Soong³

¹Cardiovascular Metabolic Disease Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; Cardiovascular Research Institute, National University Healthcare Systems, Singapore; NUS Graduate School, National University of Singapore, Singapore.

Journal of molecular and cellular cardiology

|November 3, 2024

概括

研究人员确定了VENTHEART (VHRT),一种新的长非编码RNA,对心室心肌细胞发育至关重要. 失去VHRT会损害心室功能,但不会损害心房功能,这突显了它的亚型特异性作用.

科学领域:

心脏病学心脏病学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

长非编码RNAs (lncRNAs) 在心肌细胞分化和功能中发挥作用.
关于后期心脏谱系规范和成熟中的lncRNAs的知识是有限的.
目前尚不清楚心房和心室的 lncRNA 表达的差异.

研究的目的:

为了表征一种新型的腹腔肌细胞受限 lncRNA.
研究其在灵长类人心中的保存和功能.
确定其在心房与心室心肌细胞发育中的特定作用.

主要方法:

在2,6和12周对人类多能干细胞衍生心肌细胞 (hPSC-CMs) 的单细胞RNA测序.
权重基因相关性网络分析以确定核心基因模块.
确定 lncRNA (VENTHEART,VHRT) 的CRISPR介导基因删除和随后的功能测定.

主要成果:

VENTHEART (VHRT) 被确定为人类心脏中非常丰富的lncRNA,与心室特异性基因 (MYL2,MYH7) 共同表达.
在胎儿和成人心室表达VHRT,但不是心房.
在心室性CM中,VHRT删除损害了瘤形成和心室基因程序,但不是心房性CM,在心室类CM中延长了动作潜力的持续时间.

结论:

VENTHEART (VHRT) 是第一个被识别的 lncRNA,它专门用于室内心肌细胞的规范和功能.
这一发现揭示了心室亚型特异性心脏发育的新机制.
VHRT的灵长类动物特异性保护表明,它在心室心脏发育中起着重要的进化作用.

关键词:

心脏肌细胞的特征和成熟.心肌细胞收缩的收缩一个单细胞RNAseqqq 干细胞衍生的心肌细胞.文字转录文字转录文字转录在 lncRNA 中,

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