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探索Si-Ni-San在自身免疫性甲状腺疾病中的治疗机制:网络药理学方法和实验证据
Zhiying Tan1, Gaofeng Qin2, Jianying Jia3
1Binzhou Medical University, Binzhou, Shandong, China; Binzhou Hospital of Traditional Chinese Medicine, Binzhou, Shandong, China.
Journal of ethnopharmacology
|November 3, 2024
概括
Si-Ni-San (SNS) 通过调节IL-6/JAK2/STAT3/IL-17通路,显示出对自身免疫性甲状腺疾病 (AITD) 的治疗潜力. 这种传统的疗法使Th17/Treg细胞重新平衡,为AITD提供了一个新的治疗前景.
科学领域:
- 免疫学 免疫学 免疫学
- 内分泌学 在内分泌学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 自身免疫性甲状腺疾病 (AITD) 是普遍存在的免疫媒介疾病,导致甲状腺衰竭和代谢功能障碍.
- 目前的AITD治疗有局限性,需要新的治疗策略.
- 传统的中国草药Si-Ni-San (SNS) 含有与AITD相关的抗炎和免疫调节性质的化合物.
研究的目的:
- 确定SNS用于AITD治疗的关键治疗成分.
- 阐明SNS在AITD中的有效性背后的分子机制.
- 揭示AITD干预的潜在治疗目标.
主要方法:
- 网络药理学分析以确定SNS和AITD之间的共同目标.
- 基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 途径分析.
- 超高性能液态染色学-四极飞行时间质谱学 (UHPLC-QE-MS) 用于组件分析和分子对接.
- 在体内验证使用实验性自身免疫甲状腺炎 (EAT) 鼠标模型.
主要成果:
- 网络药理学确定了32个共同目标,强调IL-6/JAK2/STAT3/IL-17途径是SNS在AITD中的行动的核心.
- 在EAT小鼠中,SNS治疗有效降低了IL-6的产生,并抑制了IL-6/JAK2/STAT3/IL-17通路.
- 在AITD中,SNS重新平衡了Th17/Treg细胞比率,证明了其抗炎机制.
结论:
- 通过调节IL-6/JAK2/STAT3/IL-17通路,SNS对AITD产生保护作用.
- 恢复Th17/Treg细胞平衡是SNS在AITD治疗作用的关键机制.
- 通过针对这些分子通路,SNS为AITD提供了一种新的治疗策略.
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