利用拉曼光谱技术用于细胞治疗生物处理
Marta H G Costa1, Inês Carrondo1, Inês A Isidro1
1iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.
Biotechnology advances
|November 3, 2024
概括
拉曼光谱为细胞疗法制造提供实时,非破坏性监测. 这种过程分析技术 (PAT) 工具提高了效率,确保了产品质量,并支持生物处理中的自适应过程控制.
科学领域:
- 生物技术和生物加工
- 分析化学 分析化学
- 细胞疗法制造业 细胞疗法制造业
背景情况:
- 细胞疗法生产需要精确监测质量和成本效益的关键参数.
- 传统的分析方法缺乏实时洞察力,阻碍了流程优化.
- 过程分析技术 (PAT) 集成对于推进细胞疗法制造至关重要.
研究的目的:
- 审查拉曼光谱在细胞治疗生物处理中的作用.
- 突出其实时,在线监控和自适应过程控制的潜力.
- 讨论其在各种制造阶段和下游过程中的应用.
主要方法:
- 整合拉曼光谱作为一个过程分析技术 (PAT) 工具.
- 在线,非破坏性测量关键参数和质量属性.
- 同时监测细胞密度,活力,代谢物和生物标志物.
主要成果:
- 拉曼光谱能够持续获取实时相关联的数据.
- 促进了简化在线监控和自适应过程控制.
- 从上游优化到下游质量控制,包括污染检测的实用性.
结论:
- 拉曼光谱显著提高了细胞治疗制造的效率和可靠性.
- 它支持严格的监管要求,并减轻与流程相关的风险.
- 这项技术有可能彻底改变先进治疗药物开发.
相关概念视频
Raman Spectroscopy Instrumentation: Overview
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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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