全转录组测序分析揭示了circZFYVE9/miR-378a-3p/IMMT轴对脂肪细胞中的线粒体功能的影响
Yanhao Qiu1, Mailin Gan1, Xingyu Wang1
1State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
International journal of biological macromolecules
|November 3, 2024
概括
这项研究揭示了圆形非编码RNA (circRNA) 和微RNA (miRNA) 如何调节脂肪细胞中的脂质代谢和线粒体功能. 确定了一个关键的circRNA/miRNA通路,影响脂肪细胞分化和线粒体活动.
科学领域:
- 分子生物学分子生物学
- 代谢研究研究 代谢研究
- 基因组学就是基因组学.
背景情况:
- 非编码RNAs,包括microRNAs (miRNAs) 和圆形非编码RNAs (circRNAs),越来越多地被认为是它们在细胞调节中的作用.
- 脂肪细胞 (脂肪细胞) 依赖精确控制脂质积累和线粒体功能的代谢恒常.
- 这些过程的失调与代谢障碍有关.
研究的目的:
- 研究miRNAs和circRNAs在脂质代谢和脂肪组织内的线粒体功能中的调节作用.
- 确定参与脂肪生成和能量代谢的特定RNA调节网络.
- 阐明这些非编码RNAs影响脂肪细胞功能的机制.
主要方法:
- 鼠标白色和棕色脂肪组织的RNA测序,以识别差异表达的mRNA,miRNA和circRNA.
- 生物信息分析用于构建circRNAs,miRNAs和mRNAs之间的调节网络.
- 使用细胞模型 (3T3-L1预adipocytes) 进行功能验证,以评估特定RNA轴对脂肪生成和线粒体功能的影响.
主要成果:
- 在白色和棕色脂肪组织之间观察到1936个mRNA,127个miRNA和171个circRNA的显著差异表达.
- 已识别的RNA网络参与了关键的代谢途径,包括线粒体生物发生,氧化酸化和酸循环.
- 功能验证了circZFYVE9/miR-378a-3p/IMMT轴,证明了它在调节脂肪生成差异化过程中的线粒体功能中的作用.
结论:
- 非编码RNAs,特别是circRNAs和miRNAs,在调节脂肪代谢和脂肪组织中的线粒体功能方面发挥着重要作用.
- 已识别的调节网络为管理脂肪细胞生物学的分子机制提供了新的见解.
- 这项研究扩大了对内源RNA对代谢健康的影响的理解,并为治疗干预提供了潜在的目标.
相关概念视频
01:28The Inner Mitochondrial Membrane
3.3K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria. In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.3K
01:45Mitochondrial Membranes
9.0K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
9.0K