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第一个三个月的循环miR-194-5p和miR-1278改善了孕前的预测

Andrée-Anne Clément¹, Cécilia Légaré¹, Véronique Desgagné¹

¹Department of Biochemistry and Functional Genomics, Faculty of Medicine and Health Sciences (FMHS), Université de Sherbrooke, Sherbrooke, Québec, Canada.

Pregnancy hypertension

|November 4, 2024

概括

在第一季度循环的microRNAs,特别是miR-194-5p和miR-1278,在与传统的风险因素相结合时,可以改善产前 (PE) 的早期检测.

科学领域:

生物标志物生物标志物
基因组学就是基因组学.
生殖医学生殖医学

背景情况:

孕前 (PE) 是导致母亲和胎儿发病的主要原因.
早期识别PE风险对于及时干预和改善结果至关重要.
目前对PE的预测模型在准确性和早期检测方面存在局限性.

研究的目的:

为了识别与PE相关的第一 trimester中的循环microRNAs (miRNAs).
评估这些miRNAs在独立队列中对PE的预测价值.
评估miRNAs和PE确定的临床风险因素的联合预测能力.

主要方法:

从两个潜在的出生队列 (Gen3G和3D) 的血样本中分析了循环的miRNAs.
差异表达分析 (DESeq2) 用于在发现队列中识别PE相关的miRNA.
为了评估预测性表现 (AUC),开发了一种包含miRNA和临床风险因素的逐步后勤回归模型.

主要成果:

73个循环的miRNAs最初与Gen3G队列中的PE有关.
在3D队列中成功复制了5个miRNA.
一个包括miR-194-5p,miR-1278,孕产妇年龄,第一季度MAP和平价的模型在Gen3G中达到0.861的AUC,在3D中达到0.842,仅仅超过风险因素.

结论:

循环中的miR-194-5p和miR-1278是早期PE检测的有希望的生物标志物.
将这些miRNA与经典风险因素结合起来,可显著提高PE预测的准确性.
这些miRNA具有很大的潜力,可以在临床实践中改善早期PE风险评估.

关键词:

生物标志物生物标志物早期怀孕查早期怀孕查怀孕期间的高血压疾病.预测预测预测孕前症孕前症利博激素是一种利博激素.这是一个微型RNA.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...