来自Aspergillus oryzae的β-galactosidase在聚乙烯醇水凝中的固定和表征
Doruk Akdoğan1,2, Ayşegül Peksel1
1Department of Chemistry, Faculty of Arts and Science, Yildiz Technical University, Istanbul, Turkey.
Biotechnology and applied biochemistry
|November 4, 2024
概括
在聚乙烯醇 (PVA) 凝中固定β-galactosidase可增强其催化性能. 这种新的配方显示出更好的稳定性和效率,使其有利于工业生物技术应用.
科学领域:
- 生物技术是生物技术.
- 酶工程是什么? 酶工程是什么?
- 生物化学 生物化学
背景情况:
- 开发新的固定酶配方是生物技术的一个关键目标.
- 酶固定增强了酶的稳定性,可重复使用性和催化效率.
研究的目的:
- 使用聚乙烯醇 (PVA) 凝来固定β-galactosidase.
- 为了优化固定化方法并描述固定化的酶.
- 为了比较固定β-galactosidase与其自由形式的特性.
主要方法:
- 通过将其困在PVA凝矩阵中来固定β-银酸酶.
- 进行了固定条件 (温度,pH) 的优化.
- 描述包括活动测定,动力分析 (Vmax,Km) 和稳定性研究.
主要成果:
- 确定最佳固定温度在40°C至50°C之间.
- 在最佳pH 7下,固定酶显示Vmax增加 (2.495 U mg-1),Km减少 (0.982 mM).
- 固定β-galactosidase在7周后保持了显著的活性,并且表现出良好的重复使用性,在三次重复使用后的初始活性为66%.
结论:
- 在PVA凝中对β-银酸酶的固定显著增强了其酶特性.
- 与自由酶相比,固定化的酶表现出更好的催化效率和稳定性.
- 这种PVA凝固定β-galactosidase具有各种工业应用的前景.
相关概念视频
01:24Oligosaccharide Assembly
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
01:25Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...