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开发一个MALDI-TOF MS模型,以区分导致出血性败血症的Pasteurella multocida菌株与其他囊群的菌株

Kelli Maddock¹, Brianna L S Stenger², Jill C Roberts³

¹Veterinary Diagnostic Laboratory, North Dakota State University, Fargo, ND, USA; College of Public Health, University of South Florida, Tampa, FL, USA.

Journal of microbiological methods

|November 4, 2024

概括

一种新的矩阵辅助激光吸附离子化飞行时间质谱法 (MALDI-TOF MS) 方法能够准确地区分出血性败血病菌株的Pasteurella multocida. 这种快速诊断工具可以帮助识别动物中严重的P. multocida感染.

科学领域:

兽医微生物学兽医微生物学
动物传染病动物传染病
质谱的应用质谱的应用

背景情况:

帕斯泰雷拉多酸菌会引起重要的动物疾病,包括牛呼吸道疾病和破坏性出血性败血症.
目前对出血性败血症的诊断依赖于病变识别和繁的血型定型或分子方法.
由P. multocida囊型B和E引起的出血性败血症,具有很高的死亡风险,并具有流行病潜力.

研究的目的:

开发和验证一种快速诊断方法,以区分导致出血性败血症的P. multocida菌株与其他类型.
利用基因组数据创建基于生物标志物的测试,用于P. multocida菌株的识别.
改善及时诊断出血性败血症,以有效管理疾病.

主要方法:

84种P. multocida菌株的基因组表征,包括出血性败血症类型 (B:2,5,E:2,5,B:3,4).
开发一种使用特定蛋白质峰值 (6419 和 7729 m/z) 的矩阵辅助激光吸附离子化飞行时间质谱法 (MALDI-TOF MS).
验证了三个自动分类模型和一个用于菌株差异化的辅助模型.

主要成果:

辅助的MALDI-TOF MS模型实现了高精度:98.2%的非出血性败血症菌株和100%的经典出血性败血症菌株 (B: 2,5和E: 2,5).
区分出血性败血症菌株 (B:2,5,E:2,5,B:3,4) 的整体准确率为96.9%.
该方法在区分引起疾病的P. multocida类型方面表现强.

结论:

MALDI-TOF MS提供了一种快速而准确的方法,用于对P. multocida分离物的初始查.
这种方法可以促进在诊断环境中早期识别出血性败血病引起的菌株.
常规实施这种MALDI-TOF MS测定可以指导进一步的表征和疾病控制策略.

关键词:

复杂的牛呼吸道疾病综合体流血性败血症是什么意思马尔代斯人马尔代斯人这种植物是Pasteurella multocida.这种病是巴氏菌病.

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