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Updated: Jun 8, 2025

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帕拉利希斯·奥利瓦塞乌斯 (Paralichthys olivaceus) GSDME介导的热死是通过多个caspases以不同的方式调节的

Kangwei Hao¹, Liming Yuan², Chao Yu²

¹CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, China; College of Marine Sciences, University of Chinese Academy of Sciences, Qingdao, China.

Fish & shellfish immunology

|November 4, 2024

概括

鱼中的热亡受气皮质E (GSDME) 分裂的调节. 卡斯帕斯激活GSDME导致细胞死亡,而其他卡斯帕斯则抑制它,提供了对鱼类热致死机制的见解.

科学领域:

细胞生物学细胞生物学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

热症是一种编程细胞死亡途径,对免疫力至关重要.
通过caspases激活气体皮质胺 (GSDM) 会启动热.
关于特里奥斯特灭的研究,特别是在鱼类中,仍然有限.

研究的目的:

为了研究鱼 (Paralichthys olivaceus) 中 pyroptosis 的激活和调节机制.
为了识别在鱼气体皮质E (PoGSDME) 裂开过程中涉及的特定的caspases.
阐明PoGSDME在细菌感染期间细胞反应中的作用.

主要方法:

分析了PoGSDME被六个鱼鱼 (PoCASP1/3a/3b/7/8a/8b) 分裂的分析.
鉴定PoGSDME功能和血膜转位的关键残留物.
检查在细菌感染期间在鱼组织中PoGSDME和PoCASP1的表达和激活.

主要成果:

PoCASP1 / 3a / 3b / 7在FEAD激活了PoGSDME,诱导了热.
PoCASP8a/8b通过在IEKD.分裂来抑制PoGSDME活动.
细菌感染上调了PoGSDME和PoCASP1,导致IL-1β和IL-18的处理.

结论:

鱼GSDME介导的热受特定的卡斯帕斯裂解部位的调节,使其能够激活和抑制.
关键残留物F2,L19和G85对于PoGSDME转移至关重要.
这项研究为鱼类的热灭菌调节提供了新的见解,特别是在免疫反应期间.

关键词:

卡斯帕塞斯 (Caspase) 是一个气体末是一个气体末.帕拉利希斯 (Paralichthys olivaceus) 是一个大橄类动物.在Teleost中,您可以使用Teleost.

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