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结肠直肠癌部位中的菌是否起源于口腔腔? 一个试点研究研究

Niels Plomp¹, Kristina Bertl^2,3, Marie-Louise Lydrup⁴

¹Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.

Clinical and experimental dental research

|November 4, 2024

概括

这项研究探讨了口腔细菌Fusobacterium和结直肠癌 (CRC) 之间的联系. 虽然在CRC患者的唾液中发现了Fusobacterium,但在瘤组织中没有检测到,这表明需要进一步研究的复杂关系.

科学领域:

微生物学微生物学
在瘤学瘤学.
遗传学遗传学是一个

背景情况:

菌细菌,特别是F.核菌,与结直肠癌 (CRC) 的发展和进展有关.
以前的研究表明,与CRC相关的Fusobacterium具有潜在的口腔来源,但缺乏确定的证据.
这项试点研究旨在开发一种方法来比较口服和CRC相关的Fusobacterium菌株.

研究的目的:

建立一种可靠的方法来比较口腔和结直肠癌 (CRC) 组织中的Fusobacterium菌株.
在CRC患者的唾液和瘤样本中调查Fusobacterium的存在和特征.
为在口腔-CRC-Fusobacterium轴上进行更大的队列研究奠定基础.

主要方法:

从牙周炎患者 (n=8) 收集的牙裂液和唾液用于Fusobacterium隔离.
从CRC患者 (n=10) 选唾液和CRC切除材料,使用培养,16SrRNA基因分析和PCR.
优化样本采集通过优先考虑唾液由于更高的fusobacterium产量.

主要成果:

在牙裂液和唾液中发现了不同的Fusobacterium物种.
通过培养,在十分之九的CRC患者的唾液中检测到fusobacterium.
16S rRNA基因分析显示,十个CRC患者中有八个的瘤组织中存在Fusobacterium,所有患者的唾液中也存在Fusobacterium.

结论:

试点发现支持口服和CRC相关的Fusobacterium之间的潜在关联.
该研究强调了Fusobacterium在口腔样本和CRC组织中的存在,尽管直接从组织中分离是具有挑战性的.
需要对更大的队列进行进一步的研究,以分析特定的Fusobacterium菌株及其在CRC中的作用.

关键词:

16S rRNA 测序的测序方法这是一种Fusobacterium细菌.这是一个PCRPCR.结肠直肠癌是什么意思文化经济学文化经济学

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