一种高效且低成本的方法,用于通过高性能液体染色学与3,3'-Diaminobenzidine衍生物结合来确定含丰富茶中的
Xiwen He1, Kai Huangfu2, Juan Zhao2
1Shaanxi Qin Yun Agricultural Science Research Institute, 714000, Weinan, China; Shaanxi Qin Yun Agricultural Products Inspection and Testing Co., 714000, Weinan, China.
Talanta
|November 4, 2024
概括
一种新的方法使用高性能液体染色学 (HPLC) 和3,3'-氨基 (DAB) 精确量化茶中的. 这种具有成本效益的技术可在富含的茶样中提供可靠的检测.
科学领域:
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
- 环境科学 环境科学
背景情况:
- 是一种重要的微量元素,具有显著的健康益处.
- 富含的茶作为的饮食来源越来越受欢迎.
- 精确量化茶中的对于质量控制和健康评估至关重要.
研究的目的:
- 开发和验证一种新的,具有成本效益的分析方法,用于量化含丰富茶中的.
- 建立一种可靠的方法来分析茶叶矩阵中的各种价值状态.
- 将开发的方法与现有的技术比较,例如原子光谱法 (AFS).
主要方法:
- 通过氧化还原反应将各种价值态转化为Se(IV).
- 用3,3'-二氨基胺 (DAB) 来衍生Se(IV),以形成Se-DAB衍生物.
- 使用高性能液态染色学 (HPLC) 用专用NH2列对Se-DAB衍生物进行量化.
主要成果:
- 通过HPLC-DAB方法,在0.01-50μgmL-1.1的范围内显示出极好的线性 (R2=0.9999) .
- 实现了低检测极限 (0.01 mgkg-1) 和量化极限 (0.03 mgkg-1).
- 观察到高恢复率 (81.2%-96.7%) 和良好的重复性 (RSDs 0.72%-8.57%).
- 结果与AFS方法具有很高的一致性,平均差异很小,为0.005mg/kg.
结论:
- 开发的HPLC-DAB方法是一种稳定,可重复和准确的技术,用于丰富茶中的量化.
- 这种新的方法为AFS等传统方法提供了一种成本效益高且灵活的替代方案.
- 该方法在处理大量样本时的效率使其适合于茶中的的常规分析.
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