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Updated: Jul 5, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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再组合酶聚合酶放大与电化学读取相结合,用于敏感和特定检测PIK3CA点突变

Vanessa Thoeny1, Eva Melnik1, Melanie Huetter2

  • 1AIT Austrian Institute of Technology GmbH, Center for Health & Bioresources, Giefinggasse 4, 1210, Vienna, Austria.

Analytica chimica acta
|November 4, 2024
PubMed
概括

一种新的测试检测了乳腺癌个性化治疗的关键PIK3CA基因突变. 这种方法使用复合酶聚合酶放大和电化学传感器进行快速的基因诊断.

关键词:
DNA-生物传感器多重复合的电化学传感器点位突变检测检测点位突变检测复合酶聚合酶放大复合酶的放大.幕打印的黄金电极使用幕打印.

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...