鉴定Burkholderia cepacia复合体:比较传统,自动化和分子方法在第三级护理中心
Priya Periaiah1, Tessa Antony1, Senita Samuel1
1Department of Microbiology, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND.
Cureus
|November 4, 2024
概括
准确识别Burkholderia cepacia复合体 (BCC) 对于患者的治疗至关重要. 传统的生物化学测试是有效的基因鉴定,而自动化方法提高了BCC的物种鉴定.
科学领域:
- 临床微生物学 临床微生物学
- 细菌的鉴定 细菌的鉴定
- 传染性疾病 传染性疾病
背景情况:
- Burkholderia cepacia复合体 (BCC) 是常见的,耐聚胺的,常常被常规方法错误识别的格兰阴性细菌.
- 需要准确和简单的识别方法,特别是在资源有限的环境中,以指导BCC感染的治疗.
研究的目的:
- 评估传统的生物化学试验,自动化方法和PCR用于识别BCC分离物.
- 分析BCC感染患者的抗生素概况和治疗结果.
主要方法:
- 通过使用常规的生物化学试验,VITEK MS,VITEK 2和groEL PCR,确定了60种抗聚素B的非乳糖发酵分离物.
- 使用VITEK AST.来确定抗生素敏感性.
- 收集了有关风险因素和治疗结果的患者数据.
主要成果:
- 传统的生物化学品和自动化方法确定了46个 (76.6%) 的BCC分离物.
- 所有方法的基因鉴定一致性为100%;自动化系统 (VITEK MS) 与生化测试相比显示出更优异的物种鉴定.
- 所有的BCC分离物都对ceftazidime敏感; 糖尿病和内置设备是常见的危险因素.
结论:
- 传统的生物化学测试可靠地用于Burkholderia的基属级别识别,与自动化和分子方法相比.
- 自动化系统增强了BCC的物种级别识别.
- BCC感染与糖尿病和内置设备有关,在适当的抗生素治疗后,康复率高.
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