相关实验视频

Updated: Jun 8, 2025

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

循环miRNA-106b-5p作为冠状动脉疾病的潜在生物标志物

Rosita Azar Bahadori¹, Dina Shabani², Elham Arjmandrad³

¹Department of Molecular Genetics, Parand Branch, Islamic Azad University, Tehran, Iran.

International journal of molecular and cellular medicine

|November 4, 2024

概括

研究人员确定了miR-106b-5p作为冠状动脉疾病 (CAD) 早期诊断的潜在生物标志物. 这种微RNA在CAD患者中表达显著升高,这表明其在临床检测中的实用性.

科学领域:

心血管研究研究心血管研究
分子生物学分子生物学
生物标志物发现发现

背景情况:

冠状动脉疾病 (CAD) 是全球死亡的主要原因.
早期诊断CAD对于有效管理至关重要.
微RNAs (miRNAs) 越来越多地被认为是它们在各种疾病中的作用,包括CAD.

研究的目的:

为了确定特定的微RNAs (miRNAs) 用于冠状动脉疾病 (CAD) 的早期检测.
研究选择的miRNAs作为CAD的诊断生物标记物的潜力.

主要方法:

对CAD患者数据集 (GSE113079) 的分析,以确定差异表达的信使RNA (DEmRNA).
计算预测,以选择与DEmRNA有显著相互作用的miRNAs.
在CAD患者和健康对照中评估miRNA表达水平.

主要成果:

根据它们与DEmRNAs的相互作用,通过计算选择了五种miRNA (miR-106b-5p,miR-20a-3p,miR-17-3p,miR-146a-5p,miR-155-3p).
与健康对照组相比,仅在CAD患者组中观察到miR-106b-5p表达的统计学显著升高 (p>0.001).

结论:

该研究在冠状动脉疾病患者中发现了miR-106b-5p的高表达.
miR-106b-5p显示为早期CAD检测的潜在诊断生物标志物具有前途.
需要进一步的研究来验证这些发现,并确认miR-106b-5p.p.的临床实用性.

关键词:

这是一个微型RNA.冠状动脉疾病是一种冠状动脉疾病.这是miRNA-mRNA的内射.qRT-PCR 是一个很好的方法.

更多相关视频

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Published on: July 27, 2016

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...