相关实验视频

Updated: Jun 8, 2025

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Published on: July 22, 2019

在癌症治疗的基本和转化方面使用CRISPR-Cas9

Maryam Samareh Salavatipour¹, Zahra Poursalehi², Negin Hosseini Rouzbahani³

¹Department of Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran ‎University of Medical Sciences, Tehran, Iran.

BioImpacts : BI

|November 4, 2024

概括

基因编辑工具CRISPR-Cas9通过精确准和修改参与瘤发展的基因,彻底改变了癌症治疗. 这项技术在治疗固体瘤和血液癌症方面表现有前途,包括增强免疫疗法.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
在瘤学瘤学.

背景情况:

基因编辑技术,特别是CRISPR-Cas9,允许精确操纵核酸序列.
基因组的改变对癌症的开始和进展至关重要.
克里斯普尔-Cas9已经成为识别和修改驱动瘤过程的基因的强大工具.

研究的目的:

审查CRISPR-Cas9技术在癌症研究和治疗中的当前应用.
探索CRISPR-Cas9在固体瘤和血液恶性瘤管理中的应用.
评估CRISPR-Cas9在推进癌症免疫治疗中的作用.

主要方法:

对科学数据库 (PubMed,Google Scholar) 和临床试验注册表 (ClinicalTrials.gov) 的文献评论.
使用关键词进行搜索:"CRISPR-Cas9"",基因组编辑"",癌症"",固体瘤"",血液恶性瘤"",免疫疗法"",诊断"",耐药性".

主要成果:

概述CRISPR-Cas9技术基础及其在各种固体瘤和血液瘤的临床前和临床研究中的应用.
在利用CRISPR-Cas9来增强免疫中介细胞疗法 (如CAR-T,CAR-NK和CAR-M细胞在瘤学中) 中取得的进展.

结论:

CRISPR-Cas9系统显著改变了某些固体恶性瘤和白血病的治疗策略.
通过CRISPR-Cas9针对关键的致癌基因,为癌症治疗提供了一种革命性的方法.

关键词:

克里斯普尔-Cas9是什么意思癌症癌症癌症癌症诊断诊断诊断诊断诊断药物耐药性药物耐药性药物耐药性基因编辑基因编辑血液的恶性瘤.免疫治疗是一种免疫疗法.固体瘤是一种固体瘤.

更多相关视频

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Published on: January 5, 2018

Production of Human CRISPR-Engineered CAR-T Cells

Production of Human CRISPR-Engineered CAR-T Cells

Published on: March 15, 2021

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...