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Updated: Jul 3, 2026

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L-carnitine对反应性氧物种减少和循环胺后小鼠的亡基因表达的影响:一项实验性研究

Majid Almasi¹, Golnaz Shafiei², Hossein Nikzad^1,2

¹Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran.

International journal of reproductive biomedicine

|November 4, 2024

概括

通过减少反应性氧物种 (ROS) 和调节卵细胞中的亡基因表达,L-卡尼丁 (LC) 保护雌性小鼠免受循环胺 (CP) 诱导的不孕症. 这种抗氧化作用可以保护卵细胞的质量免受化疗损伤.

科学领域:

生殖生物学生殖生物学
毒理学毒理学毒理学
分子生物学分子生物学

背景情况:

环胺 (CP) 是一种广泛使用的抗癌药物,已知有女性不孕症的副作用.
作为一种抗氧化剂的L-卡尼丁 (LC) 已显示出对抗药物诱导的不孕症的潜在保护作用.

研究的目的:

为了研究L-卡尼丁 (LC) 在缓解环胺 (CP) 诱导的氧化应激和雌性小鼠卵子细胞中亡的疗效.
评估LC对活性氧物种 (ROS) 水平和CP治疗小鼠卵细胞中关键的亡基因 (Bcl2,Bax,Caspase3) 的表达的影响.

主要方法:

雌性NMRI小鼠被分为四组:对照组,只有CP的,只有LC的和LC+CP的.
小鼠每天接受IP注射盐水,CP (75毫克/公斤),LC (200毫克/公斤) 或LC,其次是CP.
在超排卵后收集卵细胞,并使用光染色和实时PCR分析细胞内ROS水平和亡基因表达 (Bcl2,Bax,Caspase3).

主要成果:

在LC+CP组的卵细胞中,与CP组相比,L-carnitine的使用显著上调Bcl2基因表达 (p = 0.01) 和下调Bax和Caspase3表达 (p = 0.03,p = 0.04).
与CP组相比,LC治疗显著降低了LC+CP组的细胞内ROS水平 (p < 0.001).

结论:

L-卡尼丁有效地清除卵细胞中由环胺诱导的活性氧物种.
LC通过调节Bcl2并降低Bax和Caspase3来调节细胞亡途径,从而保护卵细胞免受CP诱导的损伤.

关键词:

环酸胺的使用方法活性氧物种.反应性氧物种.细胞灭亡 (apoptosis) 是一种死亡的过程.卡尼提因是一种碳素.基因基因基因基因

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