通过响应表面方法优化发酵条件,以生产BYST01的Aspergillus chevalieri
Shuxiang Zhang1, Zhou Jiang1, Suwen An1
1School of Life Sciences, Anhui Agricultural University, Hefei, China.
PeerJ
|November 4, 2024
概括
优化培养条件Aspergillus chevalieri BYST01显著增加了physcion的生产,一个天然的植物性杀菌剂. 这项研究提高了这种真菌在农业应用中的潜力.
科学领域:
- 微生物生物技术 微生物生物技术
- 自然产品化学 自然产品化学
- 农业科学 农业科学
背景情况:
- 拉巴的成分Physcion是中国商业上已批准的植物性真菌杀菌剂.
- 来自白的真菌Aspergillus chevalieri BYST01产生了physcion.
- 优化培养条件对于最大限度地提高真菌代谢物产量至关重要.
研究的目的:
- 为了优化培养条件,通过阿斯伯吉勒斯·切瓦利里 (Aspergillus chevalieri) BYST01.01增强生化生产.
- 为了评估Aspergillus chevalieri BYST01衍生的physcion作为植物性杀菌剂的潜力.
主要方法:
- 选基底介质,碳来源和发酵参数 (时间,温度,pH,器速度).
- 使用普莱克特-伯曼设计 (PBD) 和响应表面方法 (RSM) 的统计优化.
- 在摇瓶和5L生物反应器中进行发酵试验.
主要成果:
- 马乳糖被确定为最佳的基底介质.
- 在优化条件下 (pH 6.6,每分钟177转,28°C,30g/L葡萄糖,11天) 产生了82.0 mg/L的物理.
- 在最佳条件下,5L生物反应器在8天内达到85.2mg/L的物理量.
结论:
- 响应表面方法是优化物理生产的有效工具.
- 优化条件导致与基底平均水平相比,physcion产量增加了三倍.
- 由Aspergillus chevalieri BYST01增强的植物产量支持其在植物疾病管理中的应用.
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