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Updated: Jun 8, 2025

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
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多种类型的冷EM校准和工作流验证标准.

Daija Bobe1, Mykhailo Kopylov1, Jessalyn Miller1

  • 1Simons Electron Microscopy Center, New York Structural Biology Center, New York, New York, USA.

Acta crystallographica. Section F, Structural biology communications
|November 4, 2024
PubMed
概括

引入了一种新的冷电子显微镜 (cryoEM) 校准样本与兼容的大分子分子. 该标准有助于优化分辨率,评估仪器性能和验证冷EM工作流.

关键词:
3D重建和图像处理 3D重建和图像处理基准测试 (benchmarking) 是一种比较的方法.校准校准的时间冷电子显微镜的使用方法教育教育教育教育的教育.解决方案的解决方案解决方案的解决方案.一个粒子分析分析.一个粒子的冷EMEM.结构的确定 结构的确定培训培训培训培训培训培训培训工作流的标准工作流.

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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