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相关概念视频

Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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Pairpot:一个基于实时激光分析的数据库,专为配对单细胞和空间转录学量身定制.

Zhihan Ruan1, Fan Lin1, Zhenjie Zhang1

  • 1Centre for Bioinformatics and Intelligent Medicine, College of Computer Science, Nankai University, No.38 Tongyan Road, 300350 Tianjin, China.

Nucleic acids research
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概括

Pairpot是一个新的数据库,用于配对单细胞和空间解析的转录组学 (SRT) 数据. 它提供实时分析,从复杂的空间基因表达模式中发现生物学见解.

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科学领域:

  • * 计算生物学 * 计算生物学
  • * 基因组学 是一个学科.
  • * 生物信息学是一门学科.

背景情况:

  • * 配对的单细胞和空间解析的转录组学 (SRT) 数据为生物过程和疾病机制提供了补充的见解.
  • *现有的SRT数据库缺乏足够的单细胞和SRT (SC-SP) 对和实时分析能力,限制了生物发现.
  • *从集成的SC-SP数据中发现潜在的生物学见解需要强大的策划和先进的分析工具.

研究的目的:

  • * 开发Pairpot,一个新的数据库,用于配对单细胞和SRT数据,并集成实时启发式分析.
  • * 策划一个全面的高质量的SC-SP对和相关数据集的集合.
  • * 为了能够有效地识别空间转录基因数据中的异质域和细胞比例.

主要方法:

  • * 策划了99个高质量的SC-SP对,包括来自299个数据集的1,425,656个点.
  • * 整合了多个组织切片,并确定了单细胞和SRT数据之间的关联.
  • * 实现半监督学习用于实时启发式分析:拉索视图 (SRT域精制),对视图 (细胞比例推断) 和层视图 (3D层次可视化).

主要成果:

  • *成功策划了99个高质量的SC-SP对,并建立了协会网络.
  • * 开发并验证了SC-SP数据的实时启发式分析工具 (Lasso-View,Pair-View,Layer-View).
  • * 证明了Pairpot在识别异质空间域和推断细胞比例方面的效率.

结论:

  • *Pairpot为研究空间生物学和疾病机制的研究人员提供了宝贵的资源.
  • *数据库及其实时分析工具有助于更深入地了解空间环境中的细胞异质性.
  • *Pairpot增强了对联单细胞和SRT数据对生物发现的实用性.