通过支持液体提取和气体染色学结合联质谱法对血清多化二乙醇进行人体生物监测
Miao Liu1, Xiangyu Jia1,2, Rongfei Peng1
1Guangzhou Center for Disease Control and Prevention, Guangzhou, China.
Journal of separation science
|November 4, 2024
概括
这项研究开发了一种可靠的方法来测量人血清中的多二乙烯 (PBDE). 在90%以上的成年人中检测到PBDE,其中BDE-47和BDE-183是最常见的.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- 聚二乙烯 (PBDE) 是持久性有机污染物,具有已知的健康风险.
- 准确量化人类基质中的PBDE对于暴露评估至关重要.
研究的目的:
- 开发和验证一种可靠的分析方法来量化人类血清中的PBDE.
- 为了比较提取技术,以获得最佳的PBDE测定.
主要方法:
- 气色谱-并联质谱法 (GC-MS/MS) 用于PBDE的量化.
- 支持液体提取 (SLE) 被优化并验证为血清样本的准备.
- 方法验证包括线性,灵敏度,可重复性和矩阵效应评估.
主要成果:
- 经过验证的方法显示出良好的线性,灵敏度 (LODs 248 ng/L) 和可重复性 (RSD <11%).
- 在111名健康成年人中,超过90%的人被检测到PBDE,其中BDE-47和BDE-183是最常见的先天性病因.
- 平均BDE-47和BDE-183度分别为4.13和22.1 ng/L;男性的BDE-183水平较高.
结论:
- 使用SLE建立了一种可靠的GC-MS/MS方法用于血清PBDE分析.
- 生物监测研究证实了人类对PBDE的广泛暴露.
- 关于PBDE-183水平的性别差异需要进一步研究.
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