评估使用制造生态系统 (EcoFABs) 的Brachypodium distachyon根球中的水平基因转移
Shweta Priya1, Silvia Rossbach2, Thomas Eng1
1Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Applied and environmental microbiology
|November 4, 2024
概括
制造生态系统 (EcoFABs) 在植物根环境中有效地测量水平基因转移 (HGT). 植物阶段和压力等因素会影响HGT频率,这对于理解工程基因运动至关重要.
科学领域:
- 微生物学 微生物学
- 植物科学 植物科学
- 分子生物学分子生物学
背景情况:
- 水平基因转移 (HGT) 是根球中的一个关键的微生物过程.
- 在复杂的根球环境中研究HGT具有挑战性.
- 制造生态系统 (EcoFAB) 为植物微生物相互作用研究提供了一个可控的系统.
研究的目的:
- 为了证明EcoFABs对于量化根球中HGT频率的实用性.
- 调查影响植物相关环境中细菌菌株之间的HGT率的因素.
- 建立一个工作流程,以评估工程塑体在根球中的转移.
主要方法:
- 在一个EcoFAB中使用了三亲联系统与*Brachypodium distachyon*.
- 雇佣了*Pseudomonas putida* KT2440作为捐赠者和接受者,以及 *Burkholderia* sp. OAS925作为一个社区成员.
- 多样化的植物发育阶段,根排泄物成分,捐赠细胞数量和环境压力.
主要成果:
- 环保FABs成功地促进和测量了基因转移通过植层中的等离子体结合.
- 等离子体转移频率受到植物发育阶段,根排泄物和供体细胞度的影响.
- 从 *P. putida* 到 *Burkholderia* sp. 的已经证明的 HGT. OAS925 在根球微生物群体中.
- 环境压力显著影响了不同物种和品种之间的HGT率和效率.
结论:
- 生态FAB是研究根球中HGT动态的有效工具.
- 植物发育阶段,根排泄物,细胞密度和环境压力是调节HGT的关键因素.
- 这种工作流允许在与植物相关的环境中对工程塑体转移进行可靠的评估.
关键词:
布拉基波迪乌姆 (Brachypodium) 是一个形体.伯克霍尔迪亚 sp. 的种类.这是EcoFAB的EcoFAB.在HGTGT的基础上.伪omonas putida可以使用.结合方式 结合方式这种频率的频率是非常高的.这是一个mesocosm.根系球 (Rhizosphere) 是一个根系球.盐度 盐度 盐度 盐度 盐度三个父母组成的家庭.更多相关视频
04:09Author Spotlight: Streamlining Composite Plant Production via Agrobacterium rhizogenes-Mediated Hairy Root Transformation
Published on: June 30, 2023
2.0K
09:04Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System
Published on: May 28, 2019
10.6K
相关概念视频
01:59Plant Breeding and Biotechnology
18.8K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
18.8K
00:53Transgenic Organisms
31.0K
Overview
31.0K
02:19Export of Mitochondrial and Chloroplast Genes
3.6K
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
3.6K
02:11The Roles of Bacteria and Fungi in Plant Nutrition
35.1K
Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.
35.1K
02:50Transgenic Plants
7.2K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.2K