相关实验视频

Updated: Jun 8, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

OsPIPK-FAB是米的负调节剂,通过OsMBL1抑制揭示了米的免疫力

Ruina Zhang¹, Mengtian Pei¹, Shiyi Lin¹

¹State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops and Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Rice (New York, N.Y.)

|November 4, 2024

概括

米酸盐酸盐激酶FAB (OsPIPK-FAB) 负面调节植物免疫力. OsMBL1抑制了OsPIPK-FAB,增强了植物对爆等真菌病原体的防御能力.

科学领域:

植物分子生物学植物分子生物学
植物信号通道的信号通道.
植物病原体相互作用

背景情况:

脂氨醇信号系统对于植物生理学至关重要.
酸丁酸盐酶 (PIPKs) 合成酸丁酸二酸盐 (PIP2).
在植物信号转导中PIPKs的作用尚未完全理解.

研究的目的:

为了研究大米曼诺结合性讲蛋白1 (OsMBL1) 和大米PIPK,OsPIPK-FAB.之间的相互作用.
阐明OsPIPK-FAB在植物免疫中的作用及其通过OsMBL1.1调节的作用.

主要方法:

酵母二杂交 (Y2H) 选试验,以确定相互作用的蛋白质.
同免疫沉 (Co-IP) 和拉下测试以确认蛋白质相互作用.
在大米中进行基因删除和过度表达研究.
测量伊诺西1,4,5-三酸盐 (IP3) 含量和PIP5K活性.

主要成果:

OsPIPK-FAB被确定为OsMBL1.1的相互作用蛋白.
删除OsPIPK-FAB增强了对大米爆炸的抵抗力,而过度表达增加了敏感性.
发现OsMBL1可以抑制OsPIPK-FAB的PIP5K激酶活性,并降低IP3水平.

结论:

OsPIPK-FAB是一种新型成分,对米的模式触发免疫 (PTI) 激活有负面影响.
OsMBL1 抑制 OsPIPK-FAB,从而在调节植物防御反应方面发挥作用.

关键词:

IP3 IP3 IP3 IP3 IP3 IP3 IP3 IP3 IP3 IP3 IP3 IP3 在 OsMBL1 里面.这就是OsPIPK-FAB.在PIP5KK中,PIP5K是PIP5K.这就是PIPKs.这就是ROSOS ROS.大米大米大米大米大米

更多相关视频

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Screening and Identification of RNA Silencing Suppressors from Secreted Effectors of Plant Pathogens

Published on: February 3, 2020

相关概念视频

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

Global Regulatory Systems

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...