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Updated: Jun 8, 2025

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

Published on: December 15, 2017

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在代谢表型的工程中利用酶催化速率的优化

Zahra Razaghi-Moghadam1,2, Fayaz Soleymani Babadi1,2, Zoran Nikoloski1,2

  • 1Systems Biology and Mathematical Modeling Group, Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.

PLoS computational biology
|November 4, 2024
PubMed
概括

一种新的计算方法,即克服动力速率障碍 (OKO),通过修改酶周转数来预测代谢工程策略. 这种方法成功地使大肠杆菌和大肠杆菌中的40多种化合物的产量翻了一番,对细胞生长的影响最小.

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