绝对定量甲酸在生香中的含量.Roxb. 根体通过定量H NMR
Boonwiset Seaho1, Chatkamon Lekwongphaiboon1, Ngampuk Tayana2,3
1Department of Chemistry, Faculty of Science and Technology, Thammasat University (Rangsit Campus), Pathum Thani, Thailand.
Phytochemical analysis : PCA
|November 4, 2024
概括
一种新的定量NMR方法准确地量化了Zingiber cassumunar rhizome中的四种关键类植物. 这种技术可确保药用植物提取物的可靠质量控制.
科学领域:
- 自然产品化学 自然产品化学
- 分析化学 分析化学
- 频谱学是一种光谱学.
背景情况:
- 药用植物中活性化合物的定量确定对于质量控制至关重要.
- 植物提取物的化学复杂性对准确量化提出了挑战.
- 定量NMR光谱 (qNMR) 是一种分析自然产品的强大技术.
研究的目的:
- 开发一种定量的NMR方法,用于量化Zingiber cassumunar根茎原料提取物中的生物活性素.
- 建立一种可靠的分析方法来控制Z. cassumunar. 的质量.
主要方法:
- 使用600MHz光谱仪进行定量1HNMR (qHNMR).
- 一个内部标准被用来绝对量化四种类素.
- 方法验证包括线性,量化极限,精度和回收研究.
主要成果:
- 直接量化四种类植物素 (DMPBD,TMPBD等) 的数量. 在原油提取物中,获得了高特异性和灵敏度.
- 选择的1H NMR信号被明确地分配,并没有与其他共振重叠.
- 该方法证明了线性 (0.70-14.52毫克/毫升),低量化极限 (0.46-0.68毫克/毫升) 和高精度 (RSD <0.74%).
结论:
- 开发并验证了一种快速,准确和精确的1H NMR方法.
- 该方法允许在Z. cassumunar根茎原料提取物中同时量化四种类素.
- 这种qNMR方法适用于植物制药品的质量控制.
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