微RNA介导的网络冗余受到净化选择的限制,并有助于Drosophila melanogaster表达的强度
Aimei Dai1,2, Wenqi Lan1,2, Yang Lyu3
1State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, Guangdong, China.
Communications biology
|November 4, 2024
概括
在复合前回路 (cFFC) 中,微RNA (miRNA) 介导的调节冗余增强了基因表达的稳定性和强度. 这种冗余性,在超过75%的miRNA目标中发现,是由自然选择塑造的.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
背景情况:
- 微RNA (miRNA) 是一种非编码的RNA,可以在转录后调节基因表达.
- 转录因子 (TF) 和它们的点形成基因调节网络,通常由miRNAs共同调节成复合前回路 (cFFC).
- 在cFFCs中miRNA介导冗余的作用及其进化影响尚未得到充分理解.
研究的目的:
- 量化drosophila melanogaster中miRNA介导的cFFC冗余性的研究.
- 研究cFFC冗余与基因表达进化,稳定性和选择压力之间的关系.
- 为了阐明miRNA介导的调节冗余的功能意义.
主要方法:
- 整合来自ENCODE的ChIP-seq数据与来自TargetScanFly的miRNA准数据.
- 在Drosophila胚胎和幼虫中量化miRNA介导的cFFC冗余.
- 分析cFFC冗余性与净化选择,表达分歧和表达稳定性的相关性.
主要成果:
- 超过75%的miRNA点参与了冗余的cFFCs.
- 更高的cFFC冗余与更强的净化选择和减少物种间表达差异相关.
- 增加的cFFC冗余与增强的表达稳定性有关,特别是在压力条件下,如热冲击.
- 冗余的cFFCs主要调节进化上较老或广泛表达的基因.
结论:
- 由MiRNA介导的cFFC冗余在保持基因表达强度方面发挥着至关重要的作用.
- 自然选择有利于增加调节电路中的冗余性,以增强稳定性和保护基因功能.
- 这种冗余性有助于基因表达模式的进化稳定性.
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