精确调节特定氧气消耗率以加强Rhodobater sphaeroides的CoQ生物合成10
Bo Li1, Yan Ge2, Jianguang Liang2
1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Rd, P.O. box 329#, Shanghai, 200237, China.
Bioresources and bioprocessing
|November 4, 2024
概括
研究人员优化了使用Rhodobacter sphaeroides的辅酶Q10 (CoQ10) 生产,通过精确控制氧气水平和用介电光谱学养营养素. 这在大型生物反应器中提高了高达35%的CoQ10产量.
科学领域:
- 生物技术是生物技术.
- 微生物发酵 微生物发酵
- 代谢工程是代谢工程.
背景情况:
- 辅酶Q10 (CoQ10) 是一种重要的食补充剂,通过有氧发酵生产,主要使用Rhodobacter sphaeroides.
- 氧气的可用性是最大限度地提高R. sphaeroides发酵中的CoQ10产量的关键限制因素.
- 现有的方法缺乏对发酵参数的精确控制,阻碍了最佳的CoQ10生物合成.
研究的目的:
- 开发和实施一个精确的在线调节策略,以增强R. sphaeroides中的CoQ10生物合成.
- 建立一个量化模型,将可活细胞,细胞形态,氧吸收率 (OUR) 和CoQ10生产联系起来.
- 根据实时发酵数据优化营养养策略.
主要方法:
- 利用介电光谱 (DS) 来实时监测可行的细胞和导电性.
- 建立了一个定量反应模型,将细胞特征与氧气吸收率 (OUR) 相关联起来.
- 实施在线特定氧气消耗率 (QO2) 控制策略和基于DS的营养养策略.
主要成果:
- DS有效地监测了可行的R. sphaeroides细胞及其生理状态.
- 在0.07 ± 0.01 × 10−7 mmol/细胞/小时的优化QO2显著提高了CoQ10的生产28.3%.
- 综合在线控制策略提高了CoQ10产量35%,在50L生物反应器中达到3384mg/L.
结论:
- 精确的在线控制QO2和营养养,通过DS促进,是高度有效的增强辅酶Q10的生产.
- 开发的发酵控制策略为工业 CoQ10 制造提供了可扩展的解决方案.
- 这种方法可用于改进其他微生物发酵过程.
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