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基于质谱和光成像的药物协同作用的双模准确可视化

Jinlong Zhang^1,2, Yida Zhang^1,2, Taihe Han¹

¹State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

Analytical chemistry

|November 5, 2024

概括

这项研究开发了一种新的光探针Nap-NP-NSB,以可视化非类固醇抗炎药物和硫化 (H2S) 的协同效应. 探测器证实了H2S和纳素的存在.

科学领域:

生物医学工程生物医学工程
药理学药理学是指药理学的学科.
分析化学分析化学

背景情况:

非类固醇抗炎药物 (NSAIDs) 和硫化 (H2S) 之间的协同潜力已得到认可,但缺乏直接证据和有效的可视化方法.
精确确认协同效应是单一光检测方法的挑战.
胃粘膜损伤是与NSAID使用相关的常见副作用.

研究的目的:

开发一个集成的诊断和治疗光探针,用于可视化药物协同作用.
从开发的探测器中定量评估H2S的释放.
在细胞和动物模型中证实纳普和H2S的协同抗炎和保护作用.

主要方法:

合成了一种新的集成光探针,Nap-NP-NSB,通过将纳普与自燃间隔器结合,使H2S释放.
在体外和细胞评估H2S定量释放和对胃粘膜细胞炎症和氧化损伤的协同效应的评估.
在急性肝炎的小鼠模型中使用光和质谱成像进行体内双模成像,以可视化肝脏和胃组织中的药物协同作用.

主要成果:

纳普-NP-NSB探针成功地在体外和细胞内定量释放了H2S.
证实了H2S和纳普罗森的协同作用,显示出增强的抗炎活性和减少胃粘膜损伤.
双模成像提供了药物协同效应的准确可视化,并证实了改善的治疗结果.

结论:

开发的Nap-NP-NSB探头提供了一种结合光和质谱成像的双可视化方法,用于研究药物协同作用.
这种方法为研究NSAIDs和H2S的协同效应提供了新的策略,从而改善了治疗策略.
这些发现突显了开发具有减少副作用的向治疗的潜力,特别是对于胃保护.

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